An In - HouseOpiate EnzymoimmunoassayBased on the Syva EMIT # { 174 } Principle
نویسندگان
چکیده
Many laboratories involved in drug-abuse screening use enzymoimmunoassays such as Syva’s EMIT#{174} system. Although automation has been extensively used to reduce assay costs, further reductions can be achieved by the development of in-house reagents. We have developed our own reagents for the detection of opiates in urine by using the Syva EMIT principle and adapted it for use on an Eppendorf EPOS analyzer. The immunogen was produced from 3-carboxymethylmorphine, and morphine-3glucuronide attached to glucose-6-ptiosphate dehydrogenase was used as the tracer. Excellent agreement with Syva’s commercial assays was obtained.
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